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  • chxu
    Junior Member
    • Jan 2016
    • 1

    #1

    tophat-fusion result

    Can I use the accepted_hits.bam of "tophat2 --fusion-search" to calculate the gene expression level?

    Usually I just use tophat to map reads to genome, then calculate the gene expression level by the accepted_hits.bam. But when I need to rsearch the fusion transcript, I use the "tophat2 --fusion-search", then I got an accepted_hits.bam, could I use this bam file to calculate the gene expression level? I don't want to map the reads again using tophat2 without --fusion-search.

    Thanks!

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