Hi All,
I have a question: for a relatively "good" FastQC report, do we still need to do data pre-processing?
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45 results in 0.0032 seconds.
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What we can learn from FastQC reports
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Fusion primers
Hello,
has anyone used or knows how can I get adapter sequences and MID (multiplex identifier) tags, can I buy it or a I need to design... -
barcoded RNA adapter ligation for multiplexing
Hi All,
This is my first post here, so hello everyone!
I'm wondering if it is possible to use RNA adapter ligated to (preferably)... -
KAPA index adapter compatibility
Hello,
Has anyone used the KAPA single-indexed adapter kit with a library prep kit other than one of KAPA's? I would like to test out their newer... -
Doubt for primer removal: illumina
Hi All,
I was browsing online bioinformatics forum and stumbled onto query about primer, adapters that put me in thought about the process/pipeline... -
Ion Torrent adapter artefacts
Hi guys!
We recently began working with Ion Torrent PGM. At first we were thinking that server cuts off all adapters and barcodes and we... -
MinION adapter
Hi everybody,
I am new to this forum and I hope to start testing the MinION technology very soon with DNA/amplicons. But I have question... -
Trimmomatic adapter file vs Custom made file
Hello everyone,
I am working with TruSeq paired end data (150bp). I have a doubt regarding the adapter file provided in Trimmomatic... -
Why mapping rate is low after adapter trimming paired-end data?
I have the pipeline with the following trimming and mapping parameter:
...Code:trim_galore --stringency 5 --dont_gzip --trim1
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Can I remove index adapters after assembly?
I assembled a reference transcriptome de novo using Trinity with 2x300bp Illumina MiSeq PE reads. I used libraries from 4 individuals that were multiplexed... -
which library kit; are adapters on Illumina PCR free LT kit biotinylated ?
Hi,
I would like to prepare an enriched (custom IDT lockdown probes) NGS (Illumina platform) library that has a PCR step as late as possible in... -
Ligation of Illumina adapters to custom adapters
I would like to create libraries with the following protocol but the second adapter ligations seems not to work:
1. MseI digestion of genomic... -
How do you specify error rate in BBduk adapter trimming?
Hello,
I'm using BBduk to trim adapter sequences from my reads. From the help manual I see the editdistance and hammingdistance options,... -
finding adapters for trimming
Hi All,
I am a total newbies in this field. I want to know the trend of the community for adapter trimming steps.
I have... -
Basic question about reads pre-processing before alignment
Hi, guys,
I found I got confused with some basic concepts in reads pre-processing. I try to google them but still didn't get a clear answer....
