Hi guys!
I'm sequencing and assembling a de novo invertebrate genome of about 0,8Gb. I want to make a lane run of Illumina nextera mate-pairs,...
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Sophie's choice: Random mate-pairs or gel selection in Illumina?
Last edited by Marcela Uliano; 01-08-2015, 07:35 AM.
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Extracting unitigs from Allpaths-LG
Hello Seq-ers,
I want to extract the unitigs that allpaths constructs in its assembly process for use as high confidence sequence to correct...
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Miseq v2 PE library length for Allpaths-LG
Hello,
I'd like to use ALLPATHS-LG for de novo assembling of a fungal genome
(~20 Mbp). I will use Miseq data for the assembling....
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Haploidify in ALLPATHS-LG
Hi,
Could someone please explain to me what the HAPLOIDIFY function does in ALLPATHS-LG? I have done two assemblies with ALLPATHS-LG - one with...
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Contig coverage from ALLPATHS-LG
Hi,
I have recently done my first de novo assembly with ALLPATHS-LG. I was wondering if it was possible to determine the coverage for each of the...
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AllPaths-LG
I am trying to force AllPaths-LG to use my paired-end data with "standard" insert size of about 300bp.
As per documentation it...