Hello,
I have been using the Kapa Hyper Prep kit to try and prep DNA libraries for target enrichment.
I am using custom Y adapters and...
20 results in 0.0029 seconds.
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No amplification with Kapa kit
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Fusion primers
Hello,
has anyone used or knows how can I get adapter sequences and MID (multiplex identifier) tags, can I buy it or a I need to design... -
Low input of primers for pre optimized PCR step
Hello,
I have created a situation where I only have enough reagents to add approximately 1/5 of the primers recommended (pre optimized by Illumina)... -
Webinar Jan 25 '17: What to do when you don’t have enough genomic DNA
I apologize if this is not the correct forum to post this!
Join Lucigen for What do when you don't have enough DNA (a Webinar)
When:... -
Maximum # of copies in a cluster?
Hi all,
I am just wondering why the # of DNA copies in each cluster is capped between 1000-2000.
Is this just due to the... -
Truseq Exome Library Prep: amplification does not work
Hi,
I have 6 samples with which I am trying to make a library with Illumina TruSeq Exome Library Prep Kit (starting material was 100-300... -
Scientist/ Senior Scientist, Nucleic Acid Amplification (NGS)
Scientist/ Senior Scientist, Nucleic Acid Amplification, NGS Technologies will be responsible for development of novel, streamlined methods of nucleic... -
When to amplify libraries for RNA-seq?
Hi,
I have a relatively basic question. I am performing RNA-seq on some small tissue samples and I have constructed my first set of libraries... -
PCR amplifying microsatellites (short tandem repeats) for sequencing
Hi all,
I'm looking to amplify specific dinucleotide repeats from low input DNA (~1ng) but I'm having problems with microsatellite contraction/expansion... -
FLX+ Library Amplification Issue
We are having problems getting our long-fragment shotgun library to amplify during emPCR. Titration using 3 different cpb ratios (12, 18, and 24) yields... -
Struggling to amplify DNA with barcoded primers
Dear all
I am using the MiSeq for 16S amplicon sequencing with custom barcodes.
I use the barcoded primers on extracted... -
Targeted sequencing of ~40Mb genome
I am looking to do targeted sequencing on approximately ~40Mb of mouse genome (~2% of the genome) from low input DNA (~100pg). Equivalent to sequencing... -
Pyrotags interfering with 16S amplification
Hello!
I am having A LOT of trouble getting amplification from bar-coded primers using the Lib-L library set up. I have 9 biofilm extractions... -
best 16s region to amplify with NGS ?
Does someone have an idea of the best 16s gene region to amplify with NGS in order to assess microbial (bacterial) diversity in environmental samples... -
Innovative Solutions for Targeted Amplification in Next-Generation Sequencing
Talk at Drug Discovery & Diagnostic Development Week
Hyatt Regency San Francisco
Wednesday, Aug. 3
2:30pm - 3:00pm
ht...
