Hi everyone,
I'm using the ONT CRISPR-Cas9 targeted enrichment protocol (https://www.nature.com/articles/s41587-020-0407-5) to sequence a 18kb...
19 results in 0.0029 seconds.
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AMPure XP clumps during Cas9 library prep
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Homemade AMPure XP has some problems
Hi guys,
I have two queries about the homemade AMPure XP beads.
1) Almost in all the homemade recipe of AMPure XP beads, there... -
Low recovery from Ampure XP beads
Hello, Working on a library prep for Minion sequencing. We are working with high molecular weight DNA. Attempting to recover very large fragments (>100kb).... -
Make your own AMPure XP, cheaply
Like a lot of labs, we've replaced spin/vacuum columns with magnetic bead extraction for every part of our library construction protocols. However, the...Last edited by jwfoley; 11-01-2017, 09:14 AM. -
DNA extraction DNAdvance
During an attempt to isolate DNA from spores and save a days work I started to wonder about the contents of DNAdvance secret ingredients.
...Last edited by Strangelove; 09-12-2014, 10:34 AM. -
Ampure XP beads that do not stick to wall of plate
Hello,
I am currently having an issue that I have never seen before with my ampure bead purifications. The beads are not sticking sufficiently... -
How Ampure or SPRIselect works
We, and our NGS service providers are frequently asked about a common clean up / size selection step during library preparation. Specifically, how does... -
ddRAD size selection with Ampure beads
Hi there,
I am looking at using a modified ddRAD protocol in a genus of plants with a genome approximately 1000Mbp in size. I do not have... -
SPRI selection at high temperature or with denaturants for ssDNA?
Hi all,
I've heard that SPRI beads also work for ssDNA, and I'd like to do a SPRI bead size selection of ssDNA > 150 nt (for various... -
Magnetic bead washers
Does anyone know of a automated bead washing machine which will efficiently work with the Ampure purification beads. I don't necessarily want to buy a... -
Ampure bead clean up
Hello,
How much drying time do you normally allow when using ampure beads XP to clean up your library preps?
I've been using the... -
Removing Adapter Dimers with AMPure Beads
Can someone help?
I have adapter dimmers or some other contaminant below 200 (probably 170bp). Ultimately, my goal is to remove the contaminates... -
cDNA purification prior to shearing (for Illumina sequencing)
Hi!
I recently got the SMARTer Ultra Low RNA Kit for Illumina Sequencing, and I'm confused about their way to purify ss- and ds cDNA (they... -
weird bioanalyzer results
Hey Folks,
I'm working on a metagenomics project looking at the ITS1 region (~300bp give or take a bit) of fungal endophytes. I extracted... -
Ideas for shearing and/or size-selected FFPE DNA?
Hello,
I want to perform exome sequencing on a SOLID 5500 with DNA extracted from FFPE.
After a Bioanalyzer run, i've seen...
