Hi!
I recently got the SMARTer Ultra Low RNA Kit for Illumina Sequencing, and I'm confused about their way to purify ss- and ds cDNA (they recommend Ampure XP beads).
Now using these beads would require to get the entire other equipment required (magnet, etc). So, are there other useful and reliable ways of purifying the cDNA before shearing (like Qiagen PCR purification kit)? Does anyone know the differences in recovery and length bias?
Thanks in advance for your help!
G.
I recently got the SMARTer Ultra Low RNA Kit for Illumina Sequencing, and I'm confused about their way to purify ss- and ds cDNA (they recommend Ampure XP beads).
Now using these beads would require to get the entire other equipment required (magnet, etc). So, are there other useful and reliable ways of purifying the cDNA before shearing (like Qiagen PCR purification kit)? Does anyone know the differences in recovery and length bias?
Thanks in advance for your help!
G.
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