Greetings,
New grad student here working with a fungi with a genome that is roughly 42-50Mbs. Basically what I'm trying to do is an assembly...
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Pulling together contigs: a less stringent assembly
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Code for identifying and renaming contigs
Just completed an assembly and was wondering if there is any code around which will take my assembly, blast it, get the best hits and rename the contigs...
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how to measure the quality of PacBio data
Hi,
How to measure the quality of PacBio reads? if you have multi cells What is the tools and How to measure the quality of the read to be...Last edited by Medhat; 08-30-2014, 02:19 AM.
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Idba-ud
Anyone know how to assemble 12 files of pair end data using IDBA-UD?
Thanks,
Tom
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IDBA-UD paired end data on multiple files
I want to assemble with IDBA-UD and have no experience with it. Although my data is paired half of each pair is on one file and the other half in another...
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Help needed to polish pacbiotoca assembly with Quiver
Dear PacBio team,
I have used CA8.1 to assemble pacbio self-corrected sequences. In the wiki of PacBioToCA, it has such "After assembly,...
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Sweet spot coverage. why?
Hi everybody!
I'm a little confused with the "why" of the existence of a "sweet spot" coverage when assembling a genome....
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[Velvet,assembly] core dumped occured by runnning velvet
Deleted posted question.Last edited by matador3000; 12-26-2011, 08:37 AM.
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Rescuing mate-pair data
Hello all,
We recently had trouble with the sequencing facility we use and got a bad batch of genomic runs back that I would like to...
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Geneious Pro 5.3 Beta available now!
Geneious Pro™ continues to improve, with the beta of version 5.3 available now. Download Geneious Pro 5.3 Beta to get a sneak peak at the latest features...
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About Mosaik Assembler Consensus String
Hello everybody,
I just want to know how I can get a consensus string from the output of mosaik .ace file.
If that's impossible,...
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transcriptome assemble of SOLiD data
Anybody has suggestion for transcriptome assemble analysis on SOLiD 50bp reads? We have 50 million reads but got few contigs by using Velvet with 29 kmer....
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Another assembly tools: LOCAS (Has anyone tried already?)
I have found another assembly tools LOCAS (http://www-ab.informatik.uni-tuebing...software/locas ), but it seems there is only binary files(Redhat,...
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Overlap Graph
Dear All,
I have ~ 100000 DNA sequences in a fasta file. I am interested in finding the overlap graph of these sequences. I just want a...
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memory requirments of velvet tool (de novo assembly)
Hi,
Does someone have an idea of how much memory would velvet require for a given input of short reads? And how would it possibly scale...