Hi all!
I have generated genome assemblies for my samples and would like to find the location of coding sequences in the contigs. Using coding...
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Command line BLAST to identify genes in genome assemblies
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Help needed to prepare consensus including overhangs
I am having trouble merging my pre-trimmed fastq files to merge with the inclusion of the overhangs/nonoverlapping reads at either end. have many files...
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nguykiet44 started a topic What genes it does or doesn’t have that may influence its suitability for use as a probiotic?in GeneralWhat genes it does or doesn’t have that may influence its suitability for use as a probiotic?
Hi everyone,
I'm working on a project - Analysis of the genome of a probiotic bacterium to support commercial development. I need help determining... -
#canu error, #failed partition, #reads
Hellow Everyone,
I am currently engaged in the assembly process of Atractomorpha species using raw PacBio reads. The data from two distinct species... -
issue changing predetermined K values on SOAPdenovo2
I am currently using SOAPDENOVO2 on a supercomputer with SLURM queuing system to perform a de novo assembly from FASTQ paired-end files with genomic DNA... -
Nextflow workflow for ONT reads QC, Assembly, Polishing and Evaluation
Can anyone suggest if there is any comprehensive snakemake or Nextflow workflow for ONT reads QC, Assembly, Polishing and Evaluation? -
Specify Bowtie2 to align a mate pair only to the same RefSeq
Hey everyone,
I am doing exome sequencing of multiple genes, of which some share great homology.
Some of the read pairs don't share... -
Assemble Anaeroplasma species genome from whole genome sequencing
Hi everyone,
I have a fasta file containing reads generated by PacBio whole genome sequencing of a feces sample from mouse. I need to extract... -
MaSuRCa: Super reads failed - kUnitigLengths.txt is of size 0
Hello,
I am trying running an assembly with MaSuRCa but am getting an error at the step: "Computing super reads from PE". ... -
Hi-C
Dear all:
We have doing Hi-C sequencing of a few eukaryotes and during the assembly process, we are finding chromosomes to be joint together.... -
Separe viral genomes to observe deletions
Hi!
I have a mixture of viral genomes assembled against the reference genome, how can I separate them to be able to observe the cut-off points... -
Resources to learn genome assembly workflow for small genomes (like viruses)
I have sequencing data of a few samples of a `DNA genome virus`. I'd like to learn `de novo assembly` of the `short reads`, making scaffolds from it,... -
SOAPdenovo2 long run
Hello all,
I’m trying to assemble a plant genome (this is my first time). I have 100bp PE reads (106G forward and 106G reverse).... -
Metatranscriptome read mapping yielding a highly left skewed ORF TPM distribution
I'm going to jump right into the problem and put details below for clarification:
What are the possible causes of mapping high quality... -
Discovering the world of the Long-read nanopore Sequencing
Nanopore sequencing technology is an emerging and a promising sequencing technology, which promises high sequencing throughput, low cost and longer read...
