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SNP Bacterial Genomes per year
I am looking at SNP trees for bacterial genomes (mostly N. gonorrhoeae, S. aureus and E. faecium) and I am following outbreaks in society and on hospital...
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CNV detection with single-read data (bacterial genome)
I am planning to do a CNV calling for the first time and I've got Illumina single-read data (50bp). Libraries were not sequenced in paired-end because...
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RiboZero kits
Hello,
After looking on the web, I haven't been really successful in finding information about the difference among the several rRNA removal...
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Input BAM files for Cufflinks
Hello,
Can I use the BAM file obtained after mapping of RNA-Seq reads using CLC Genomics Workbench as input for Cufflinks, in order to...
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Viral metagenomics sample with a high concentration of bacteria
Hi all,
I have analyzed a total of 7 samples coming from the bacteriophage fraction of human faeces. Purification included resuspension of...Last edited by chariko; 12-04-2015, 04:02 AM.
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Identifying orthologous genes between two bacterial strains
Hi,
I currently am looking at two different strains of the same bacteria. I have RNA-seq data and reference genomes from NCBI. What I want...Last edited by aprice67; 11-30-2015, 11:51 AM.
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List of all sequenced Bacteria and their GC content
Hi everyone,
How is the best way to get a list of all names (full taxonomy if possible) of bacterial strains that were sequenced, plus their GC...
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RNAseq - Alternatives to SeqScript?
Howdy all,
Looking for lower cost alternatives to SeqScript. I realize a big portion of the cost is tied up in the Ribo Zero depletion...
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mapping bacteria metatranscriptome to genomes
Hello! I'm pretty new to rna-seq data analysis and I'm not sure about how proceed with it.
I have bacteria metatranscriptome reads from environmental...
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common mRNA isolation kit for bacteria and fungi
Dear Folks,
Just curious to know about any mRNA isolation protocol available today that works for both bacterial and fungi. I have an NGS experiment...
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Weird SNPs - Assembly Error or Mapping Error?
Hello all,
I need some help interpreting what I'm seeing in my assembly data.
I'm doing some variant calling in a small...
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Simulating a genome with known SNPs
Does anyone know of a way to generate a simulated bacterial genome with known SNPs and indels relative to a given reference? I'd like to be able to generate...