In a wig file,
I would like to encode + values as watson and - values as crick. However,
wigtobigwig doesn't allow overlapping...
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strand info in wig format
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Chrom Sizes for Ensembl Reference
i want to convert BAM to BigWig for ensembl reference genome (Homo_sapiens.GRCh38.81.fa to be specific) using the following work flow.
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Extraction of BigWig data from UCSC
Hi,
I have a set of big wig files for ChIP-seq data and im looking to extract the values over a set genomic interval. However I have over 170 different...
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Chromosome sizes don't match
Hi,
I'm having a heck of a time finding the chromosome sizes for mm9 that match the mm9 Bowtie pre-built indexes on their website. I...
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byte range enabled server for NGS data?
I use the UCSC genome browser to view NGS data. The browser needs to point a url to a server where data is stored in a binary (bigWig) format. I’ve...
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Karyotype view of bigwig/bedgraph file
Hi everyone. I couldn't find a similar thread on here for my question, so forgive me if this has already been discussed elsewhere.
I have...
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Strand Rule Problem via rseqc in single end reads
Built an rna-seq pipeline for paired end reads.
Got single end data and had to revise.
Only problem I have now is the rseqc...
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Got a problem while converting wig to bigwig
I got my .wig file from MACS and normalized it(all values divide by 4.7)
When trying the code:
...Code:grep -v ^track IP_norm.wig | wigToBigWig
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Converting a fixed step wiggle file to a bigWig.
I have a fixed step wiggle file which I'm trying to convert to a bigWig file but I'm not getting anywhere. Here are the first few lines of the fixed step...
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cghub ?
I have 2 questions for folks using CGHUB ( https://cghub.ucsc.edu/ ) , the BAM warehouse for TCGA (The Cancer Genome Atlas - at URL: http://cancergen...Last edited by Richard Finney; 03-26-2013, 11:06 AM.
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View and manipulate bigWig files
Dear experts,
I have downloaded the FAIRE-seq 'Signal' files that are in bigWig format from the UCSC Open Chromatin downloads to use them...