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We work with a lignocellulose-degrading microbial consortia. Within a time-series experiment of an enriched culture, we perform...
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Question: Metatranscriptome binning
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Multithread MyCC
Dear SeqAnswers community,
Can someone please tell me how to multithread MyCC? I don't see any options in the documentation. Not sure if...
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Post binning
Are there any binning programme available to do binning just on the basis of coverage and GC % ?
I used SPAdes to generate assembly and...Last edited by sk_minu; 07-31-2016, 12:52 PM.
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Clustering/Binning Metagenomic contigs with MyCC and 16S sequences
I have tried to use MyCC (http://www.nature.com/articles/srep24175) on samples with varied diversity and I always get very good accuracy between number...
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Metagenomic contig binning and 16S with MyCC
I have tried to use MyCC (http://www.nature.com/articles/srep24175) on samples with varied diversity and I always get very good accuracy between number...
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Resolve species/strain in metagenomic bin
Hi all,
I am trying to recover microbial draft genomes from metagenomic contigs.
I have used several assembly and binning...
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shorter contigs after binning and reassembly
Hello friends, maybe you can help me with this.
I have a coassembly of two metagenomes, from which I can retrieve the contigs that belong...
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Blasting contigs against reference database
Apologies if this has been covered elsewhere, couldn't find a satisfactory answer easily....
The problem: I have hi-seq 2500...
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Binning expression data along with capturing gene identifiers
Got the answer from Bioconductor support
Thanks to johannes.rainer
https://support.bioconductor.org/p/64409/#64433
Hello,...
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NORMALIZED tetranucleotides frequency
I want to do a PCA of metagenomic contigs based on tetranucleotide composition. Commonly I read in the literature the use of NORMALIZED tetranucleotide...
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Emergent self-organizing maps from 4mer frequencies (binning metagenomic contigs)
Does anyone here have experience in assembling genomes from metagenomic contigs? I have found that this protocol, which is optimized for AMD datasets,...
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Introducing BBSplit: Read Binning Tool for Metagenomes and Contaminated Libraries
BBSplit is a tool that bins reads by mapping to multiple references simultaneously, using BBMap. The reads go to the bin of the reference they map to...Last edited by Brian Bushnell; 09-16-2014, 08:29 AM.
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Code to bin reads that have been mapped
Basicaslly the idea we have is to map our metagenomeic reads to a file containing all isolated genomes from the environment. Then remove the mapped reads...
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Why does UCSC genome browser NOT using spatial index?
UCSC genome browser uses binning strategy to retrieve genes more efficiently than simply creating a B-tree on the start and end coordinates. This certainly...
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Can anyone explain BAM indexing algorithm to me?
I have been trying to understand how the BAM index works by reading the SAM specification (v1.4, the newest version) and even samtools' source code. However,...