Does anyone here have experience in assembling genomes from metagenomic contigs? I have found that this protocol, which is optimized for AMD datasets, works really well for one of our samples (I got two >90% complete genomes out of it). However, I haven't seen much success with our other samples, which are larger and represent perhaps slightly more complex communities. So anyway, if somebody here has experience with this, I'd love to hear some suggestions especially concerning what training input parameters should be tweaked, and how. Thanks.
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by seqadmin
The field of immunogenetics explores how genetic variations influence immune responses and susceptibility to disease. In a recent SEQanswers webinar, Oscar Rodriguez, Ph.D., Postdoctoral Researcher at the University of Louisville, and Ruben MartÃnez Barricarte, Ph.D., Assistant Professor of Medicine at Vanderbilt University, shared recent advancements in immunogenetics. This article discusses their research on genetic variation in antibody loci, antibody production processes,...-
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Next-generation sequencing (NGS) and quantitative polymerase chain reaction (qPCR) are essential techniques for investigating the genome, transcriptome, and epigenome. In many cases, choosing the appropriate technique is straightforward, but in others, it can be more challenging to determine the most effective option. A simple distinction is that smaller, more focused projects are typically better suited for qPCR, while larger, more complex datasets benefit from NGS. However,...-
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10-18-2024, 07:11 AM -
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