Hi All,
I'm trying to get an idea of the processivity of Nanopore. For instance, do the pores reliably read the full length of 5 kb DNA...
9 results in 0.0029 seconds.
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Nanopore: Input cDNA Length vs. Read Length?
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Two cDNA Peaks on Bioanalyzer
I used 200 ng total RNA input and the KAPA HyperPrep Kit to generate cDNA libraries. Usually, our libraries look like Sample 1 in the attached pdf, but... -
Role/Importance of adapter contamination in cDNA libraries
I have created cDNA libraries from Drosophila melanogaster mRNA using the KAPA mRNA HyperPrep kit. The size distribution on the Bioanalyzer looks good,...Last edited by patkrat; 08-12-2017, 08:03 AM. -
Good way to make cDNA libraries for one-embryo RNA-seq
Hi!
I am a undergraduate student and planning to conduct single-embryo RNA-seq.
The developmental stage of samples are various, from blastula... -
How do people know if a SRA dataset is generated by normalized cDNA library or not?
I am looking for RNA-seq data generated from non-normalized cDNA library. However, many datasets I have looked at do not show this information. How should... -
dscDNA or not?
I have now run through the clontech low input RNA protocol again for generating dscDNA and obtained the following results with the high sensitivity dsDNA... -
Wang, 'Low Cost Library Construction...': Low Concentration
Hi All
I'm using the updated Wang et al Library construction protocol [Universal adapters and Indexed Primers + Universal primers for Enrichment].... -
Using cDNA as input for TruSeq: Anyone tried this?
We have a cDNA sample that we would like to use to make a TruSeq library. We tried Nextera already, but Nextera due to how it tagments doesn't give us...Last edited by sweetph3; 10-09-2013, 10:52 AM. -
cDNA library for Clip-seq at Illumina platform
Hi all,
Has anyone working on Clip-seq any advice to prepare cDNA library from low yield of RNA input?
We are planning to employ Illumina...
