Hi all,
I am currently setting up two QIAseq PRO targeted DNA panels for the analysis of DNA from human FFPE samples in my lab. We are using...
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Qiagen CLC Workbench QIAseqPRO targeted DNA panels - commercial vs own customization of templates
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CLC main workbench: search local BLAST DB field 'name'
Hi, I'm using CLC Main Workbench and need some advice on using the search facility.
I have a local Blast database generated from genome contigs,...
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RRBS mapping efficiency in CLC vs Bismark
Hi
we are working with RRBS data from bull sperm samples. previously we used Bismark mapper for pig data and the results were very good...
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Annotation for "upregulated" genes
Hello, I'm a very beginner at NGS.
I ran RNA-seq and performed De Novo Assembly & RNA-seq analysis using CLC Genomics Workbench. The...
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Region of zero coverage
Dear all,
I have been sequencing some samples and found something that is new for me.
After sequencing using Miseq, I usually align reads...
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CLC results - open source visualization
Dear all,
I have a little problem and I was wandering if someone of you could help me.
I am using CLC genomics workbench to analyze my sequencing...
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Uploading MiSeq mate-pair in CLC Genomics Workbench
Dear all,
I'm trying to import MiSeq FastQ files to CLC Genomics Workbench (version 4 point something), and I want to make sure that I'm...
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Transcriptomics analysis
Hi all,
I am a newbie in the field of transcriptomics analysis and I want to get more familiar with it. I know I should first normalize...
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microsatellite query
Hello wise people!
I have approx 10,000 microsatellites that I obtained from some assembled dna of a non-model organism. I selected a subset...
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Conversion file .dat (Blast2GO) into GOA file
Hello everybody,
I have a old Gene Ontology file from Blast2GO (.dat) made by a previous collegue and I would like to convert it in a GOA...
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Detecting SNPs, SNVs using CLC genomics wqorkbench
Hi everyone!
I am quite new to this field, and as such have a lot of questions but I don't know how to frame it...so please excuse me as...
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Minimum contig & Coverage
Hello,
I have some 454 sequences reads from a cDNA library for a novel organism (non-sequenced genome).
I have processed...
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RPKM and RNAseq data normalization
Hi Everyone,
I am working on a set of bacterial RNAseq data (generated using Illumina Hiseq 2000 sequencer) for differential...
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Howdy!
Greetings from Texas. I'm a PhD candidate in the field of evolutionary genetics, and I am here to do some population genomics with Illumina HiSeq data....
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estimating paired end distances
I am getting some unexpected results when I try and estimate the insert size on paired end and mate pair data. I am using CLC Genomics WB and doing a...