Dear all,
We are planning to use human/mouse intestinal tissues/biopsies for V4 microbiome sequencing. I am worried that the large amount...
30 results in 0.0027 seconds.
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16S sequencing tissue - host DNA contamination
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Subset trinity transcripts to remove contamination
I have a set of trinity-assembled transcripts that are supposed to be eukaryotic, but it appears that bacterial contamination was an issue during the... -
Poly(TA) reads in NGS libraries
Hey all,
Recently, our lab is generating RNA-Seq and ChIP-Seq libraries that contain very high amounts of reads containing a poly(AT) sequence.... -
Determine species composition in short-read data
Hi,
I am wondering the best approach for testing for contamination in short-read sequence data from host/environmental genomes which were not the... -
Contamination resources
Hi All,
I'm working with a library with significant bacterial contamination, and I've spent a lot of time trying to remove it without much... -
MiSeq fungal contamination
Hello,
We detected some kind of biofilm on the Miseq manual sipper and have reported to Illumina support. The reply from the supposed Illumina... -
Bioanalyzer, empty wells show profiles.
Dear All.
It has occurred in the last couple of run that empty wells in the bioanalyzer actually showed a RNA profile (indistinguishable from a... -
Barcode Contamination - iChIP
Hi everyone,
I have attempted to perform indexing-first ChIP (iChIP) as described here: http://www.ncbi.nlm.nih.gov/pubmed/25103404. The... -
Tools to determine sequence contamination in Methyl-Seq data ?
Hi,
I have a question regarding determining sequence contamination from Methyl-Seq experiments.
As we all know, for Methyl-Seq... -
rRNA contamination due to GL000220.1
We are carrying out RNAseq on some human tissues and have been facing rRNA contamination issues.
We first carried out library prep with the Ribozero... -
index contamination in multiplexed run
Hi all,
I have an issue with adapter/index contamination in a multiplexed lane:
I multiplexed 8 samples in a single HiSeq... -
bases after adapter contamination
Hi everyone,
I was recently experimenting with simNGS, the NGS simulation tool of EBI (https://www.ebi.ac.uk/goldman-srv/simNGS/), to create... -
Contamination between samples run on the same lane
Hi colleagues,
I found a strange effect when sequencing several samples on Miseq. The reads from each sample contain about ~0.1% of reads from... -
Media a potential source of DNA contamination?
Hi Everyone,
I am going to try to make this long story short and sweet. I have an environmental bacterial isolate that I would like to... -
how to detect contamination or sample preparation artifacts in sequencing results
Hi,
Is there any tool (apart from fastQC) to detect all primers/adapters(contaminants basically) in my raw fastq file that needs to be...
