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  • roll
    Member
    • Aug 2009
    • 38

    #1

    how to detect contamination or sample preparation artifacts in sequencing results

    Hi,

    Is there any tool (apart from fastQC) to detect all primers/adapters(contaminants basically) in my raw fastq file that needs to be removed before mapping?
  • JackieBadger
    Senior Member
    • Mar 2009
    • 385

    #2

    Comment

    • roll
      Member
      • Aug 2009
      • 38

      #3
      Thanks. I start using this and now it complaints about libraries.

      No search libraries were configured at /Downloads/fastq_screen_v0.4.2/fastq_screen line 119.

      I assume it refers to the contamination list. I have the cocntaminant file from the fastQC but it is not clear to me where to mention this list.

      I use the following line in my conf file.

      DATABASE Adapters /Volumes/bowtie2_index/

      Can you please advise if by library it means contamination list and where i should mention the libraries?

      Comment

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