Hi,
I have an alignment of human data (a small bam file with less than 1M reads), and would like to calculate and plot the coverage around...
13 results in 0.0048 seconds.
-
Coverage around genomic feature - large amount of data
-
Nucleotide coverage if >5 reads
Hi everyone,
I have generated BAM files after mapping my Illumina reads to a reference genome. Now I want to know how much of the reference... -
Metabarcoding coverage question
Hello,
I want to use MiSeq to metabarcode arthropod samples. I am producing 300 bp amplicons from 130 samples with approximately 600 specimens... -
Calculating coverage from a SAM file
My goal is to determine the ratio of raw sequencing reads which produced one contig to sequence reads which formed a different contig.
... -
Calculating percent covered per chromosome
Hi All,
I would like to calculate the percent of a chromosome covered at various depths. In other words, to answer how much of chrY was... -
Coverage Calculation for Whole Genome Sequencing on GA II X
Hello Everybody. How does one calculate the coverage on a paired end Illumina GA II X run? My fast qc statistics for the reads are as follows:
... -
Finding genome coverage using random reads
Thank you for looking at my question. I am trying to solve this homework question.
Consider the problem of sequencing genome by... -
estimating sequencing coverage for multiplexed mrna-seq
I am planning a library construction approach for one lane of 100nt paired end Hiseq sequencing. It's a (non-model) population-level study so I'll need... -
General coverage questions for NGS
How many flow cells do I need for sequencing a human genome with 30X converage (paired-end)?
Does the number of reads related with the number of... -
GATK DepthOfCoverage at high depth
I have a sample on which custom capture was done. Many of the baits have >2000x coverage.
When running DepthOfCoverage initially I never... -
Experimental design based on coverage needed
I would like to do RNAseq and DNAseq on human samples and need 500x (RNAseq) and 30x (DNAseq) coverage. How do I determine if I can multiplex and then... -
Single stranded coverage from sam/bam
Hi all,
Does anyone know of a fast, handy way to calculate coverage of the forward and reverse strands in a genome independently?
... -
mouse miRNA seq with SOLiD, help with coverage needed desperately!
Hello,
I am a novice member with a novice question
I plan to conduct a mouse miRNA sequencing project with the SOLiD...
