Hello,
I am a novice member with a novice question
I plan to conduct a mouse miRNA sequencing project with the SOLiD platform. The platform I would use gives 250million reads in a single run, and there are 590 mouse miRNAs listed on miRbase as of now. I have an option of multiplexing my samples, with the 16 barcodes available presently. The major headache I have now comes from having to decide on the number of samples I need to run in order to get a decent biological information without compromising the coverage .
I could not find a proper literature to help me calculate the minimum coverage I would need for mouse miRNA seq. Does anyone have an idea? How can I calculate my coverage from the information I mentioned above? or what exactly do I need to know?
I would be very grateful should someone explain to me in simple terms the issue of coverage in the SOLiD (mouse) miRNA sequencing context.
thanks in advance
I am a novice member with a novice question
I plan to conduct a mouse miRNA sequencing project with the SOLiD platform. The platform I would use gives 250million reads in a single run, and there are 590 mouse miRNAs listed on miRbase as of now. I have an option of multiplexing my samples, with the 16 barcodes available presently. The major headache I have now comes from having to decide on the number of samples I need to run in order to get a decent biological information without compromising the coverage .
I could not find a proper literature to help me calculate the minimum coverage I would need for mouse miRNA seq. Does anyone have an idea? How can I calculate my coverage from the information I mentioned above? or what exactly do I need to know?
I would be very grateful should someone explain to me in simple terms the issue of coverage in the SOLiD (mouse) miRNA sequencing context.
thanks in advance
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