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  • Experimental design based on coverage needed

    I would like to do RNAseq and DNAseq on human samples and need 500x (RNAseq) and 30x (DNAseq) coverage. How do I determine if I can multiplex and then also choose between single-end and paired-end to get this coverage?

    Do I simply calculate #reads required from the following?
    coverage = (read count x read length)/genome size
    30 = (read count x 36)/3GB
    read count = 90GB/36
    read count = 2.5GB ??!!

    Thanks in advance

  • #2
    Correct. Obviously, longer reads would help :-).

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