Hi there,
I have some questions regarding CLC Bio vs. Trinity for de novo RNA seq assembly. When I was told how to process my data by a postdoc...
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CLC Bio vs. Trinity for de novo transcriptome assembly
Last edited by nurgling; 04-08-2017, 12:07 PM. -
DNASTAR Releases Lasergene 14 Software Including Three New Applications
MADISON, Wisconsin – September 30, 2016 – DNASTAR® announced the release of Lasergene 14 today, which includes a broad range of improvements in DNA,... -
Designing primers on assembly: bizarre problem
This is a bizarre problem that happened a couple of years ago to myself and is now happening to a colleague.
The aim is to design primers... -
Trinity Chrysalis died with exit code 9
Hi, I'm running Trinity v 4.4.5 to perform some de novo assemblies using PE data. I get an error at the ReadsToTranscripts step, as follows:
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deNovo assembly. High percentage of improper pairs.
Hi,
I'm trying to obtain the best de novo transcriptome assembly for my data. I pooled together all the reads from my different samples... -
Target prediction between complementary RNA-seq and Small-RNA-seq data sets
Hi everyone-
Short background: I have Illumina sequence data on mRNA and microRNA from the same samples. Theoretically these two separate... -
How to calculate RPKM of de novo assembled sequences
I got some single reads produced by illumina ,and it's assembling sequences by Trinity; I want measure those seqs RPKM（which indicates expression... -
Any experience with eXpress?
Hello.
We are doing some environmental RNA sequencing from an Antarctic time series. I have some prelim data from a HI Seq run, full multiplexed...
