Trinity Chrysalis died with exit code 9

[flobpf]

Hi, I'm running Trinity v 4.4.5 to perform some de novo assemblies using PE data. I get an error at the ReadsToTranscripts step, as follows:

COMMAND: <path>/trinity/20130225--GCC-4.4.5/Chrysalis/ReadsToTranscripts -i both.fa -f <path>/chrysalis/bundled.fasta -o <path>/chrysalis -strand -max_mem_reads 1000000
Died with exit code 9
Exiting.
core file size (blocks, -c) 0
data seg size (kbytes, -d) unlimited
scheduling priority (-e) 0
file size (blocks, -f) unlimited
pending signals (-i) 191873
max locked memory (kbytes, -l) unlimited
max memory size (kbytes, -m) 8097152
open files (-n) 16384
pipe size (512 bytes, -p) 8
POSIX message queues (bytes, -q) 819200
real-time priority (-r) 0
stack size (kbytes, -s) unlimited
cpu time (seconds, -t) 7200
max user processes (-u) 1024
virtual memory (kbytes, -v) unlimited
file locks (-x) unlimited

followed by:

Error, the Chrysalis process failed:
Error, cmd: <path>/Chrysalis -i both.fa -iworm <path>/inchworm.K25.L25.fa -o <path>/chrysalis -cpu 10 -min_glue 2 -min_iso_ratio 0.05 -glue_factor 0.05 -weldmer_size 48 -min 200 -dist 500 -max_reads 200000 -sort_buffer_size 20G -max_mem_reads 1000000 -strand 1 -paired -reads_for_pairs both.fa -butterfly<path>/Butterfly/Butterfly.jar -no_cleanup 2>&1 died with ret 65280 at <path>/trinity/20130225--GCC-4.4.5/Trinity.pl line 1476.

at <path>/trinity/20130225--GCC-4.4.5/Trinity.pl line 1101
main::run_chrysalis('<path>...', 'both.fa', 200, 500, 'RF', 'both.fa') called at <path>/trinity/20130225--GCC-4.4.5/Trinity.pl line 930

Does anyone know what exit code 9 means, and what I can do to fix the issue?

Thanks!

[flobpf]

Solution?

It may be that this error is related to memory usage. Doubling the memory seems to have fixed the problem. Trinity ran all the way through to the end.