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Small RNA seq degradation
Hi, in your opinion, for a small RNA seq experiment, the percentage of the reads that map on rRNA could be a "degradation index" of the library?... -
mRNA traces on Bioanalyzer
I know it's a discussed argument, but I'd like to have an opinion about my Bioanalyzer traces.
I extracted total bacterial RNA from food samples,... -
Degraded RNA
Greetings,
I am trying to generate transcriptomic libraries from brain tissue. I used a Ribo-zero gold kit to deplete my total RNA. I ran a... -
Quantitate study of RIN vs degradation rate for poly-A purification libraries?
Hello,
I am interested in using RNA-seq data to identify fusion genes. To do this you need a certain number of reads supporting a given fusion,... -
Identifying degradation products in smallRNASeq dataset
Hi all,
I am currently working with my first small RNA dataset (generated on Illumina GAII). The preprocessed dataset contains about 16... -
RNAlater and Illumina Sequencing
I'm about to harvest mouse tissue for my undergrad RNA-Seq gene expression project on an Illumina HiSeq. I can either flash freeze the tissue on liquid...
