Greetings,
I am trying to generate transcriptomic libraries from brain tissue. I used a Ribo-zero gold kit to deplete my total RNA. I ran a pico bioanalyzer analysis and found out that most of my samples are degraded. I have very strong bands that are less than 200nt. I would like to use the Scriptseq v2 kit to prepare my libraries for illumina sequencing (100 paired ends). I was wondering if anyone would advise whether to continue this process or consider alternatives at this point. Thanks!
Joe
I am trying to generate transcriptomic libraries from brain tissue. I used a Ribo-zero gold kit to deplete my total RNA. I ran a pico bioanalyzer analysis and found out that most of my samples are degraded. I have very strong bands that are less than 200nt. I would like to use the Scriptseq v2 kit to prepare my libraries for illumina sequencing (100 paired ends). I was wondering if anyone would advise whether to continue this process or consider alternatives at this point. Thanks!
Joe
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