Dear Seniors,
I'm a newbie in field of bioinformatics and learning everything on my own by following tutorials available online. Right now, I'm...
12 results in 0.0031 seconds.
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NCBI WGS submission: Need Help
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DeNovo ChIPseq and Trinity
Hi all,
I'm trying to assemble some ChIPseq for RNAPolII data in eukaryotes de novo, and I'm using Trinity as per the post doc in the lab's... -
Velvet assembler ambiguous calls
Hello,
I'm using Velvet assembler and I've a little doubt.
After reading Velvet documentation I know that one gap in output assembly...Last edited by gmarco; 06-23-2014, 12:21 AM. -
Two related transcriptomes: merging but avoiding fake fusion transcripts
Hi. I'd like to discuss a situation that has been partially discussed in this thread:
http://seqanswers.com/forums/showthread.php?t=33... -
How to compare de novo and reference mappings in SNPs analysis?
Hello. I am a beginner in this field and I have a question, sorry if it is obvious !
In SNPs analysis (by Stacks software) with FASTQ sample... -
Transcript redundancy in denovo assembly
Hi There,
I am analyzing de novo assembled transcriptome data from a plant and see there are various loci, each with one to multiple transcripts.... -
Canonical direction and assembly
Hi,
I'm looking at Contrail and trying to understand what "Canonical Sequence Direction" means. In Contrail the canonical sequence... -
FPKM determination of de novo transcripts
I've used a de novo assembler on some pe rna seq data. However, I'd like to determine the FPKM of the transcripts that have been assembled. I've mapped... -
Targeted de novo assembly
Hello,
I have an interesting problem I am looking for some advice.
I have whole genome resequence PE illumina data and I... -
Removing primers
Hey all,
I am new to using gsAssembler. I want to do an assembly of cDNA sequences. However, they have primers attached to it. I saw an... -
Problems with de novo transcriptome assembly using 454 Titanium series
Hi,
I am trying to do a de novo transcriptome assembly for a non-model organism using 454 GS FLX Titanium series. I have 656878 reads with... -
SOAP denovo assembly results
Hi,
I was able to assemble more than 100 million reads to contigs using SOAP. That was cool, as all other tools ran into memory issues.....
