Hey all,
I am new to using gsAssembler. I want to do an assembly of cDNA sequences. However, they have primers attached to it. I saw an option called 'Trimming database for De-Novo assembly, and provided the list of primers as an input. However, when I check the output assembly, several contigs still have those primers. How do I make sure that these are removed before assembly( I have a .sff file).
Also, I want all the contigs after assembly. The AllContig.fna in gsAssembly is not listing all the contigs ( like the singletons). How do I obtain all the contigs from the assembly?
Thanks in advance for the help,
Regards,
I am new to using gsAssembler. I want to do an assembly of cDNA sequences. However, they have primers attached to it. I saw an option called 'Trimming database for De-Novo assembly, and provided the list of primers as an input. However, when I check the output assembly, several contigs still have those primers. How do I make sure that these are removed before assembly( I have a .sff file).
Also, I want all the contigs after assembly. The AllContig.fna in gsAssembly is not listing all the contigs ( like the singletons). How do I obtain all the contigs from the assembly?
Thanks in advance for the help,
Regards,
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