18 results in 0.0022 seconds.
-
How to find secreted protein tags for my DEGs list
I have found the Differentially expressed genes between breast cancer datasets using DESeq2 further I want to find the secreted protein tags for my Differentially... -
how to treat read-through in mapping for differential expression analysis
hi all
I got 2x150 reads that I used to perform denovo assembly using Trinity, with Trimmomatic option on. Problem is, that nearly 35-40% of reads... -
Question about using limma for differential analysis
For the Differential analysis I'm using "limma" R package. I'm using RSEM log2 transformed counts data from XENA browser. A matrix "U"... -
Individual-level RNA-Seq differential expression analysis with paired sample design
Hi all, I'd appreciate any thoughts that will help us with the experimental design for our project.
Our experimental design involves collecting... -
TPM and FPLM problem?
Hi
I have used Illumina hiseq 2000 to sequence two samples (two organs of a plant) without replication. I know that without replication I can not...Last edited by Ahmadiut; 06-24-2016, 04:08 AM. -
Differential analysis of different cells
Dear all, I would to know if it is correct to do a differential analysis of different cells, for example adult staminal cells from one mouse and embrional... -
edgeR doesn't produce output files
Dear SEQanswer community,
I run edgeR analysis for 8 samples (4-leukemia patients, 4-healthy donors)
I want to perform normalization,... -
questions about differentially expressed genes analysis
Hello! I an preparing the samples and designing the analysis scheme for RNA-seq. I want to use two groups of samples to do differential analysis, do all... -
miRNA annotation from miRBase 21
Dear all,
I just checked miRBase 21 has released and try to look their annotation .gff for quantification. I noticed, in gff has more counts... -
Any suggestion of tool for differential expression test for my situation?
I am trying to get differentially expressed genes for this experiment*
where one condition has one biological replicate (two samples) and the other... -
differential expression of Novel miRNA and its validation
Hello all,
Could someone suggest me that how to discriminate control vs treatment of predicted novel miRNA by mirdeep2 ??? in order to validate...Last edited by unique379; 05-23-2014, 12:00 AM. -
Diff Genes from Cuffdiff output
Dear All,
i have Rna seq data drug treated in 4 diff time interval. my cuffdiff output gives more than 20000 significant genes. I used... -
coef vs contrast in edgeR glmLRT
I thought I understood what the contrast= option in glmLRT means as opposed to coef= (which just looks for DE wrt the index=1 sample, presumed to be the... -
Greetings from the Land of Oz
Greetings,
I am an inspiring computer scientist who wishes to emphasize his research in computational biology. I look forward to getting... -
rDiff - error while getting gene expression
Hi everybody,
I was wondering if anybody could help with an error message I am receiving for the differential isoform analysis software...
