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  • miRNA annotation from miRBase 21

    Dear all,

    I just checked miRBase 21 has released and try to look their annotation .gff for quantification. I noticed, in gff has more counts (mature) than miRNA reported in site ??? how and why i do not understand !!!!

    counts shown in site : Mus musculus (1193 precursors, 1915 mature) [GRCm38]

    counts from gff annotation: Mus musculus (1128 precursors, 2046 mature)

    why ?? i have fear that it can affect in differential expression analysis. Because gff counts are not equal to real miRNA from the site. is it ??

    please someone describe it ???? and tell me should i worry about it ?? either i use genome or miRBase reference for mapping.

    Hope anyone has noticed it before ??

  • #2
    cross posted: https://www.biostars.org/p/114478/

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