Hello,
we are using tag-wise dispersion on a data set that has the format of 3 controls, 3 treatments with substance X such that they are...
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edgeR Dispersion Calculation across treatments
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Variance modelling in DESeq2
Hi there,
My apologies if this is a stupid question. I've just moved into RNA-seq analysis, and am trying to figure out some issues....
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Expression variability between 2 groups of genes
Hi, I'm analyzing GTEx data (read counts, ~30 patients, one tissue) to check if expression variability is different for 2 groups of genes (I'm interested...
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Mean-variance relationship in RNA-seq
hey all,
i am plagued by a problem regarding the mean-variance relationship in RNAseq experiments, hopefully you can help me with this.
...
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Understanding DESeq2 dispersion estimates
I construct a toy dataset with
...Code:> m Control Case GeneA 3891 4591 GeneB 69543 72122 > colData
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estimazing size factor in DESeq
Hi,
I am using Deseq to analyze my data. I have three replica for each of the two conditions.
When I am running the script...
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Strange Dispersion and Density Plots in cummeRbund
Usually by the time I get to cummeRbund (having run TopHat, cufflinks, cuffmerge and cuffdiff) I see something in the dispersionPlot that looks at least...
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DESeq high withing group dispersion
Hi forum,
I am analyzing counts data from Tophat bam followed by HTSeq.
I have 11 biological replicates for group 1 and 10 biological...