29 results in 0.0023 seconds.
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NGS library enrichment question
I have a Hi-C library with P5 and P7 adapters. I would like to enrich for fragments containing a locus-of-interest by capture and extension with a biotinylated... -
Help with genomation
Hi,
I am trying to use genomation to look at signal enrichment at one specific locus. Their "learning" examples work for me,... -
which library kit; are adapters on Illumina PCR free LT kit biotinylated ?
Hi,
I would like to prepare an enriched (custom IDT lockdown probes) NGS (Illumina platform) library that has a PCR step as late as possible in... -
GeneTrail 2
I've really started liking this tool called GeneTrail 2. There is a REST API for it and I'd like to take full advantage of it in R. There is already a... -
Motif enrichment in MEME - CentriMo
Hello,
I have a list of differentially epxressed gene for which I would like to see whether they are enriched over background (non DE genes) for... -
Cycle number? Indexing/enrichment NEBNext
I'm preparing dual-indexed libraries using NEBNext Ultra Library Prep for targeted resequencing on Illumina MiSeq and can't decide on the number of PCR... -
Low Frequency Mutation Detection by NGS
See new technology for gene variation enrichment using PCR prior to NGS:
http://bioaccent.org/cancer-sciences...e-in-press.php
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PGM: No loading on 316v2 chip
Hello,
I ran a 316v2 chip today for the 2nd run of an initialization. My chip has 2% loading.. could this be a templating or enrichment... -
pathways differential expression analysis with KO
Hello!
I assigne K0 onthology to my genes from bacteria metatranscriptome. Now I'd like to carry out a differential expression analysis. Obviously,... -
Enrichment + bisulfite conv + amplification + seq
Hi there,
I want to enrich my samples with MethylMiner™ from invitrogen. And I also want to use one bisulfite conversion kit followed... -
GO enrichment analysis for a non-model system
I have data from a few RNAseq experiments on a non-model eukaryote, in which I have assembled transcriptomes with Trinity and analyzed expression with... -
TopGo, factor of 1 means
Hi,
I'm trying to do GO enrichment analysis using topgo, but i've encountered a problem, read below. what does factor w/ level 1 mean?... -
loading custom annotation on topgo
Hi,
I'm stuck on loading my customize annotation on topgo using R. I have a .txt file containing list of genes and their GO Ids.
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Library prep kits and MYbaits compatibility
Hi all,
I'm wondering if anyone has any experience using Illumina's TruSeq NANO DNA kit with MYcroarray's MYbaits. The NANO kit has been... -
GO term enrichment with non-model organism (I have the GO terms for every locus)
Greetings. I am looking to do a GO-term enrichment analysis on a set of genes from an annotated genome whose GO terms are not loaded into the standard...
