PGM: No loading on 316v2 chip

[jhalpin]

Hello,

I ran a 316v2 chip today for the 2nd run of an initialization. My chip has 2% loading.. could this be a templating or enrichment failure? My QC percentage was 7.24%. Low, but we've had runs like that succeed.

The first run for the initialization was also a 316v2 chip and had no problems.

Screenshot attached.

Any ideas appreciated

Thanks,

Jessica

[jhalpin]

So I had a sudden flashback to mixing the melt off solution and thinking it didn't look "sudsy". It's entirely possible I put water in instead of tween, since the bottles are exactly the same. Has anyone else done this? Were your results similar?

[jhalpin]

In case anyone is wondering, it wasn't the tween. I tried to run this pool again yesterday and got the same results. Measured the pool by qubit (should have been ~30ng/mL) and it was too low to read.