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MiSeq Cycle 1 Possible Clustering Failure
We have run the same library on two different machines using a MiSeq V2 300 cycle kit and it has failed in cycle 1 both times. We are following the same...
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All ChIP Seq Reads failing to Align to reference genome
Hello NGS community,
I'm new to NGS analysis. I have some chipseq data for a transcription factor. The sequencing facility provided data as CRAM...
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low quality amplicons
Hi all,
I recently received the results back from a MiSeq PE-300 amplicon run, and found really weird results that we cannot explain. ...
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MiSeq Run, Problem with Index reads
I just ran a miseq run using NEBNext Ultra DNA prep, (the bio analyzer results looked fine prior to sequencing).
We sequenced 6 samples...
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loading bam file failure on IGV
Hi everyone,
I tried to find the reason for a long time. But couldn't solve my problem.
When loading my sorted bam file (with bai...
I was able to run a sample from failure #1 of the post-zymo cleanup on Agilent 2100 Bioanalyzer...