14 results in 0.0035 seconds.
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Significant variation in FastQ File Sizes - Fragmentation Issue?
Hi all, I’m pretty new to NGS and library prep. I just completed library prep and WES for a pilot of 12 FFPE DNA samples, and got back Fastq files that... -
Trimmomatic error reading Fastq files
I'm trying to run Trimmomatic on some shotgun sequencing paired end files and while most of them are working (so I know the code isn't the problem), a...Last edited by lkazen; 10-12-2024, 09:33 AM. -
empty fastq files after Miseq is done
Dear all,
I am new in this field and I done my first sequencing using MiSeq. I checked the files and found that all generated fastq.gz files are... -
Question: Need to create a small artificial SRA file for unit testing
Hi folks. I'm looking for a way to create an SRA file from an artificial fastq file for the purpose of unit testing a SRA file loader. The resultant SRA... -
demultiplex FastQ files based on read names
Hi everybody,
my name is Fabian Roger and I am a PhD student in Ecology at the university of Gothenburg.
I am at a stage... -
How to know whether its Read1(Forward) or Read2(Reverse) from fastq contents.
As per fastq file description on wikipedia(https://en.wikipedia.org/wiki/FASTQ_format) Illumina Sequence Identifier format as :
Case A. Standard...Last edited by vaibhavvsk; 12-23-2015, 03:26 AM. -
adapters
how to get universal adapters for illumina 1.5 for the cutting the adapters site of fastq file -
Sorted BAM read count >2x total FASTQ count
This might be a silly question but it is bugging me and I need the answer. I have a set of paired end FASTQ files that contain about 30 million reads... -
nested for loop to concatenate fastq files
My pooled PE RNA-Seq data was demultiplexed by the sequencing facility, so the data I receive is a directory with sub directories for each sample that... -
bwa mem for compressed fastq files
Hi Everyone,
I want to use bwa mem to align my fastq files to the ref genome.
My fastq files are .bz2 compressed. Something like... -
merging two fastq files from exome sequencing
Hi ,
I have 50 samples,each having paired end reads,Do you know of using "cat" command of merging paired end reads for each sample... -
fastq to bam
Hi!
Can anyone tell me is it possible to convert a .fastq file to a .bam file? How to convert or which tool can I use?
Thanks in a... -
Fastq file format for paired end sequences
Hi,
I have got my sequencing data from a sequencing-core-facility. It has been done with illumina paired end sequencing. But the reads... -
Find Common Reads between two FASTQ files
Dear experts,
I have two fastq files contains RNASeq reads of two technical replicates (at the level of re-run the sequencing machine twice)...
