As per fastq file description on wikipedia(https://en.wikipedia.org/wiki/FASTQ_format) Illumina Sequence Identifier format as :
Case A. Standard...
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How to know whether its Read1(Forward) or Read2(Reverse) from fastq contents.
Last edited by vaibhavvsk; 12-23-2015, 03:26 AM. -
NextSeq500 early results
Hi all,
We replaced our Hiseq1000 with a NextSeq, largely due to the expense of the HS maintenance contract and the long run time. Our... -
processing Fastq files from HiSeq2000 single end and RNAseq analysis
Hello all,
i apologise in advance for asking some basic questions
i have recently started working on RNA-Seq. i have fastq... -
SRA to fastq
Hi,
I'm trying to convert an sra file to fastq. i downloaded the sra toolkit and ran fastq-dump, here's what i got:
... -
Mothur and fastq file format
Hi all.
I am completly new at NGS. I have just got back my datas from a first run of 454 pyrosequencing. We want to get into metagenomics... -
where is real problem, tophat2 code or my fastq files
Dear FOlks,
I am so new, an early stage researcher.
I am using TopHat2 to map the reads, I guess, I am fulfilling all the requirements,... -
troubleshoot with tophat2 or problem with my fastq files
Dear Folks,
I am using TopHat2 to map the reads, I guess, I am fulfilling all the requirements, additionally I give
/usr/local/bin/tophat2... -
Velvet + meta-velvet:which fastq format for paired-end infos?
Hi,
I've been doing some metagenomic assemblies using meta-velvet. I've been using paired-end data for mor reliable assemblies. However, when I...
