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NEB Fragmentase mitogenome advice
Hi, I am currently working with mitogenome sequencing of small mammals. I have amplified 5 PCR amplicons of the mitogenome around 4-5kb fragment lengths....Last edited by frascom; 06-07-2019, 07:26 AM. -
Tapestation quality peaks show unwanted larger fragments.
Hi all,
I used Covaris to shear human genomic DNA at a set size of 300bp and 200bp respectively. This is to sequence my samples...Last edited by mzahir89; 08-19-2015, 02:20 AM. -
Fragment analysis; microsatellite or SSR/STR
Has anyone been using next gen or preferably illumina for fragment analysis.
Right now my project are running multiplex for Sanger 3130xl machine...
