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Multi-marker metabarcoding: how much length difference will give marker bias?
Hello SEQanswers community! I want to sequence bacterial, fungal, plant and arthropod libraries on one MiSeq run from the same faecal samples. I know... -
Options for poorly fragmented library.
Hi all,
I've been working for some months to collect samples and build libraries for an absolute RNA-seq method, which is preferably PCR-free.... -
Bioanalyzer/TapeStation fragment sizes larger than agarose gel
I am preparing libraries for GBS, and although the agarose gel image is as expected (i.e. the bulk of fragments between 200 and 400bp), TapeStation result...Last edited by cpylant; 10-27-2017, 08:56 AM. -
TruSeq Nano Library kit produced largger fragemnt sizes: 600-1100 bp
This is my first time in using the TruSeq library Nano LT preparation kit, and also I was using ezRAD method, digesting the DNA samples using Ecor I and...Last edited by Dongmei_MPI; 10-02-2016, 01:57 AM. -
Novice help: ddRAD for population genetics
Hi all,
We're working with the plant genus Diascia. There's no reference genome, and there isn't data on the overall genome size. We want... -
Tapestation quality peaks show unwanted larger fragments.
Hi all,
I used Covaris to shear human genomic DNA at a set size of 300bp and 200bp respectively. This is to sequence my samples...Last edited by mzahir89; 08-19-2015, 02:20 AM. -
Truseq: insert size <100bp complications
Hello community,
I currently have a designed custom amplicon panel with insert sizes around 100bp which for the most part work fine. I'm noticing... -
Miseq v2 PE library length for Allpaths-LG
Hello,
I'd like to use ALLPATHS-LG for de novo assembling of a fungal genome
(~20 Mbp). I will use Miseq data for the assembling.... -
How to find library fragment size for ENA raw sequence data
Hello!
My first post, I couldn't find the answer by searching, so here goes:
Does anyone know if ENA submissions are required... -
Nextera problem：Fragment size control
Hi, guys. I'm trying to construct a PE500 library from 50ng genomic DNA by using the Nextera transposase. However, the fragment size always concentrate...
