15 results in 0.0024 seconds.
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Read depth extraction
Hello. I wanted to extract the read depth information from my vcf file. Can anyone share their her/his experience in doing this. My vcf format is like... -
Methylation-sensitive restriction enzymes for GBS
Hi all!
I need help in selecting a restriction enzyme for GBS. Elshire's protocol for GBS calls for methylation-sensitive enzymes (They... -
Mabuhay from the Philippines
<first post didn't post for some reason. ho-hum. This is my 2nd time to introduce myself.>
Hello everyone! I'm Pauline, from the Philippines,... -
Mabuhay! Hello from the Philippines!
Just signed up in SEQanswers today. I've been lurking around this forum and finally decided to join to learn more and of course, contribute.
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Bioanalyzer/TapeStation fragment sizes larger than agarose gel
I am preparing libraries for GBS, and although the agarose gel image is as expected (i.e. the bulk of fragments between 200 and 400bp), TapeStation result...Last edited by cpylant; 10-27-2017, 08:56 AM. -
Validating SNP calls quality
Hi,
I'm working with GBS data and i used Stacks and ipyrad to call de novo based SNP's, apart from the quantity of SNP's called by each software... -
GBS: eliminating adpater contamination in library for Illumina platform
We're in the process of trying to create our own GBS libraries for various species. The step we're having the most trouble with right now is optimizing... -
ddRAD/GBS lib conc on HiSeq 3000/4000 ?
Hi all,
Has anybody any advice on the "best" concentration (pM) to load a ddRAD or GBS libraries on a HiSeq 3000/4000 ? ddRAD... -
Uneak pipeline in gbsv2
Hello Everyone,
Currently I am Woking on the pipeline of GBSv2 and my query is regarding the same.
AS I am new to this field.... -
epiGBS
Nature methods epiGBS article:
Please notice our Nature methods epiGBS article on epiGBS:
epiGBS: reference-free reduced representation... -
nextRAD publication
We at SNPsaurus quite frequently get asked about publications using nextRAD. Of course, it take a while to go from genotyping data to publication! Today... -
NGS library prep method for RRS/GBS/RADseq of degraded/ancient DNA?
Hi,
I am looking for the best NGS library prep method for RRS/GBS/RAD sequencing of degraded/low/template/ancient DNA ? Any good protocol... -
Designing P1 P2 adapters for RAD-seq
Hi,
I am doing some research on how to design the optimal P1 and P2 adapters for RAD-seq, and had a couple of questions.
I know... -
Filtering SNPs by coverage in HapMap format
I have data GBS derived from the UNEAK pipeline and I'd like to use the count.hmc file to remove SNPs from my HapMap.hmn file which fall below a given... -
How many individuals per population?
G'day Everyone,
I wasn't sure where to post this question; my apologies if I'm in the wrong spot.
I recently read this paper...
