Hi!,
I need to make coverage plots/GC bias plots with inferred sequences from Illumina paired-end sequences. Someone here posted that he or she...
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Coverage plots with inferred fragment sequence from PE reads
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HiSeq rapid run overclustering
Hello,
On the Illumina website, it says that the optical raw cluster density for PhiX runs is 850K-1000K clusters/mm2. But, what is the functional... -
AT rich region depletion
We are having some trouble with our ChIP-seqs: we seem to be completely blind to AT rich regions. When I check the GC distribution in the ChIP and Input... -
NEBNext vs Illumina TruSeq ChIP-Seq library prep
Hi, I was wondering if anyone did a comparison between ChIP-Seq data obtained through NEBnext and Illumina Truseq library prep.
We're... -
GC Bias on Illumina Platforms
Does anyone know if Illumina sequencing is still expected to be biased against GC rich sequences?
In doing some bacterial genome resequencing...
