Hi!,
I need to make coverage plots/GC bias plots with inferred sequences from Illumina paired-end sequences. Someone here posted that he or she wrote a tool for this purpose. I am wondering if anyone remembers or knows such a tool.
To make it clear: I have 300 bp inserts and I did 2 x 50 bp Illumina sequencing. Thus, middle 200 bp of the fragment is not sequenced. However, I can infer these middle sequences from mapping results to human genome. It’s something I can figure out from SAM file myself, but I think there are available tools that would be more convenient for me to use.
Thank you for the help.
I need to make coverage plots/GC bias plots with inferred sequences from Illumina paired-end sequences. Someone here posted that he or she wrote a tool for this purpose. I am wondering if anyone remembers or knows such a tool.
To make it clear: I have 300 bp inserts and I did 2 x 50 bp Illumina sequencing. Thus, middle 200 bp of the fragment is not sequenced. However, I can infer these middle sequences from mapping results to human genome. It’s something I can figure out from SAM file myself, but I think there are available tools that would be more convenient for me to use.
Thank you for the help.
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