Is there really no way (program, tool) to tell if your BAM files have amplicon reads?
How is everyone doing it? determining a priori and...
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Find out if you have amplicon reads.
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Alignment of reads obtained from HaloPlex HS target-enriched sequencing
Hello everyone,
I have reads that are obtained from HaloPlex HS target-enriched sequencing (Illumina MiSeq platform).
As per my understanding,...Last edited by vandana8; 06-20-2017, 10:54 PM. -
Agilent Haloplex DNA Ligase test
We bought this kit for DNAseq from Agilent (Haloplex Target Enrichment System) to use in Illumina Miseq. Right now, we have DNA Ligase (that came with... -
Recommendations for Haloplex on MiSeq
Dear users,
We are planning to run Haloplex custom library samples on a MiSeq.
After reading some older posts of 2013, I understand... -
Custom Haloplex Kit Issues
Hello all,
Just out of interest has anybody had any issues with using Haloplex Library prep, particulary the custom kits? We are really... -
biased mutation calling/false negatives from HALOPLEX datasets?
Hi everyone,
I'm wondering if someone has an opinion/experience with that. Using Haloplex, considering the frequent occurence of restriction... -
Analysing MiSeq data with SureCall
Hi everyone!
I was wondering if anyone on here has used SureCall for detecting low level mutations in a sample?
I'm a bit confused... -
Haloplex and SureCall
Has anyone had any experience using SureCall software provided by Agilent to analyse Haloplex data? I have installed this but is is supposed to connect... -
ForeignMan started a topic Handling duplicates and other biases in Haloplex enriched targeted sequencing datain GeneralHandling duplicates and other biases in Haloplex enriched targeted sequencing data
Hello everyone,
we're starting to work with deep sequencing data of target regions that were enriched by Agilent's Haloplex kit (Illumina... -
Capture or amplicon?
Hey all, I'm looking to sequence human exons across 189 genes totalling about 700kb. Is there anyone who's tried both target capture and amplicon sequencing...
