Dear users,
We are planning to run Haloplex custom library samples on a MiSeq.
After reading some older posts of 2013, I understand that people sometimes struggled with clustering and low percentages passing the filters, when using pools of 8 or 10pM. Is there any improvement since?
Haloplex pools still can contain some adapter leftovers, what about spiking with PhiX?
Can you give recommendations about the pM of Haloplex pool to load for optimal results, and the percentage of PhiX to use?
Thanks in advance,
Arjen
We are planning to run Haloplex custom library samples on a MiSeq.
After reading some older posts of 2013, I understand that people sometimes struggled with clustering and low percentages passing the filters, when using pools of 8 or 10pM. Is there any improvement since?
Haloplex pools still can contain some adapter leftovers, what about spiking with PhiX?
Can you give recommendations about the pM of Haloplex pool to load for optimal results, and the percentage of PhiX to use?
Thanks in advance,
Arjen
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