Hello all,
i apologise in advance for asking some basic questions
i have recently started working on RNA-Seq. i have fastq...
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Any suggestion for Chip-Seq analysis?
Hi! All,
I'm a newbie for Chip-Seq analysis.
As you guys already know, there are a bunch of software to analyze Chip-Seq data,...
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RNA-Seq throughput
Hello everyone!, I have a dumb question, but I didn't found nothing about that.
I want to do RNA-Seq paired-end with Hiseq2000 to grape mRNA (to...
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NexteraXT sequencing primers sequences
hi, I want to run NexteraXT libraries on the HiSeq and would like to order my own stocks of sequencing primers HP10, HP12 and HP11 rather than the Illumina...
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150bp-1.3kb insert size PE on HiSeq
I have a library that I am running on a HiSeq2000 for PE-100 reads. The fragment sizes range from 150bp to 1.3kb. I can't do much about this range since...
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Minimum dsDNA detection by NGS machine
Hello,
I am new here and to NGS. I prepared some multiplex DNA libraries with Illumina TrueSeq Kit. I submitted each sample/index at 10nM,...
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Index read problem
Hi everyone, I'm a new user of Hiseq, this is a first time that I'm running a multiplex run and I have some concerns about Index read. Until the index...
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Illumina HiSeq 2000 publication
Hi,
I was wondering if there is any published study using HiSeq 200 data. I am looking for some sample RNA-Seq data from the TruSeq chemistry.