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Source of control human/mouse RNA from blood
I'm trying to source total RNA from human or mouse blood. Does anyone know a source or has a lab that's willing to make some?
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fuellen started a topic Need a pointer to a dataset with matching animal+human for two different tissuesin GeneralNeed a pointer to a dataset with matching animal+human for two different tissues
Hello,
is anyone aware of publicly available data with at least 8 samples describing gene expression as follows, each time for 2 conditions, so...
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suitable aligner for human RNA-seq
Hello,
Human RNA-seq dataset was generated from Illumina HiSeq 3000 with 2X100 cycles run.
The first step is making alignment...
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Shotgun Metagenomics data analysis
Hi,
I am new to shotgun metagenomics analysis. I have a project on the human gut microbiota. I know about MG-rast but I want to analyze...
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RNA -> cDNA libraries on PacBio
I am at a point in my project where it would be exceedingly useful to perform RNA-seq on full human transcripts on the PacBio platform. Can anyone point...
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Looking for Mouse and Human Neuron RNA seq. data
Hello All,
Can somebody point to raw Transcriptome sequence data for mouse and Human neuron? I wish to assemble that using TopHat.
Than...
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Predicting promoter sites from NGS experiments
Hi,
is it possible to predict promoter sites from NGS experiments? Given a set of RNAseq experiments (human, triplicates) of several biological...
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miRNA analysis
Hi all,
I am working on microRNA seq (human) and I was wondering if there is a recommended tool for whole analysis i.e alignment, annotaion...
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1000 genomes human reference
Hello,
I'm using hg19.
As you may know when you perform variant calling, reference variation allele may be biased due to...
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mutation detection but has to exclude known polymorphism?
Recently got a project on human data. It is easy to find out all the mutations. But how do I exclude all the know Polymorphisms, any approach? Thanks...
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Finding orthologous sequence in human
Hi, all,
I have a bunch of mouse sequences and would like to find the orthologous human sequences. How to do this?
I found this...
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How to estimate age of a whole genome seq?
hi I need to estimate the evolutionary age of a whole genome sequence. that is, I altered the reference human genome in certain aspects and trying to...
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Unusual small FPKM Cufflinks values
Hi,
Recently I obtained some expression results for a standard RNAseq anaysis in human samples. I'm surprised by these results because...