15 results in 0.0021 seconds.
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Significant variation in FastQ File Sizes - Fragmentation Issue?
Hi all, I’m pretty new to NGS and library prep. I just completed library prep and WES for a pilot of 12 FFPE DNA samples, and got back Fastq files that... -
Download human genome (hg19) custom range
Hello!
I am attempting to download custom coordinates say (chrX:66765028-66765050) from the hg19 human genome reference.
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Viral gene in data
hi,
I have some RNA-seq data for human cell line samples and one of them expresses a viral gene, I would like to map and quantify the data... -
Download Human Genome
I want to download assembled human genome from nbci site to calculate some statistics on it. I found GRCh37 in the link below
http://www.ncbi.... -
"Dynamics of the Microbiome on Health and Disease"
Advances in next-generation sequencing and computation have elucidated the impact of human genomic variation; however, the impact of this variation is... -
UCSC human conservation data
I am using GenomeGraphs and want to plot conservation scores alongside human gene regions. In the vignette, they use yeastCons1. Where would I get the... -
Is cufflinks still useful if you have a reference genome?
Sorry, the actual heading for my post should be:
If you have a high quality genome annotation, should you always still use cufflinks.... -
samtools mpileup and bcftools command lines to compare individual differences?
Which parameters are you using for samtools mpileup and bcftools to compare individual SNP differences?
Could you write your commands?... -
short read qualities for various NGS machines
Hi,
I am looking for the DNA sequence coverage profiles for the human genome for various different sequencing machines, such as Illumina... -
Why so many unaligned reads inspite of "similarity" of any 2 human genomes?
Hi,
I apologize for my potentially "silly" question. However I've been playing around with data from the 1000genomes project,... -
sequence coverage profile/gaps for various sequencing machines
Hi,
I am new to the NGS community, and I am wondering if anyone can help me out. I am looking for the DNA sequence coverage profiles for the human... -
SOLID data processing by using BFast
Hello,
I am beginning in the NGS processing world, and I have to deal with SOLID reads. I have carefully read the manual and supplement... -
Big Genomes and Velvet
I was asked to get an estimate of how much memory it realistically takes to run Velvet to assemble a large genome, e.g. human genome size. If anybody... -
RepeatMasker report of GRCh37
Hi everyone,
I recently used RepeatMasker to analyze the DNA sequences of broken fragments of some cancer patients' chromosomes. I finnaly... -
Download promoter sequence db for human genome?
Hi all!
Sorry for the slightly off topic question, but....
Does anyone know where I can find a downloadable database of...
