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Sequence transposon flanking region
I am new to NGS first of all. I would like to sequence transposon flanking region, thus during PCR amplification step, instead of using primers supplied... -
Illustra Triple Prep Kit
Has anyone used the GE Illustra Triple Prep Kit for RNA isolation for subsequent sequencing on the Illumina GAIIx? Did you have any issues with low recovery... -
PICARD reports only unmapped reads
Dear all,
I am new to the new gen seq and have a bit of a problem using PICARD.
Basically, I have Illumina paired-end sequencing...Last edited by saskak; 03-25-2011, 02:15 AM. -
Which would be best for transcriptome analysis?
Hi,
I am a newbie to NGS and am going to work on transcriptome analysis. Which technology would be best for this, Solid or Illumina GA?...Last edited by pinki999; 10-12-2010, 12:25 AM. -
Solid vs Illumina Genome Analyzer
Hello Friends,
I am supposed to decide on whether to opt for Solid or Illumina GA for my project (transcriptome analysis). Can you guys...Last edited by pinki999; 10-07-2010, 12:16 AM. -
Insert size for paired end sequencing for identification of structural variation
Dear all,
Does anyone have any experience of the ideal fragment size for paired end sequencing looking at structural variation in whole genome... -
Massive parallel amplicon sequencing on Illumina GA
My questions are about amplicon sequencing.
I would like to sequence numerous PCR products on the Illumina GA.
I have about 50 amplicons...
