21 results in 0.0026 seconds.
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Low-plex pooling: how bad will this combination be?
Hello! This is my first post to SeqAnswers, but I have perused the forums before, so before I start describing my problem, hello to this fantastic community....Last edited by msanthropologist; 12-17-2024, 09:45 AM. -
Indexing -bowtie2, aligning - STAR and FATAL ERROR
Ciao,
I'm a Bioinformatics newbie encountering an issue with STAR alignment. I downloaded the mm10 genome and indexed it with Bowtie 2. When... -
My Unique Dual Indices with Nextera adapters, dont bind with any library preparation method
Hello everyone!
We designed Unique Dual Indices, with both nextera adapter sequences and truseq so we can see which performs better, in the... -
Demultiplexing failures in MiSeq run
Hi All!
I am sequencing (on a MySeq platform) a custom library containing 4 i7 indexes. In principle, it works, but when I look on demultiplexing... -
Samples artificially grouped by i5 inline barcodes, in Illumina HiSeq 2500
Hi,
I am using single digest RAD-Seq for analysing the population structure of a protist species, using the dual barcoding by i7 index barcodes... -
non-equimolar pooling to normalise read depth in libraries with different genome size
I'd like to sequence libraries of very different genome size on a single MiSeq run.
Eight bacterial samples with 4.5Mb genome size
... -
HISAT2: advantages of build index using annotated transcripts
Hello,
after reading the paper, I am still not sure about what is the advantage of building an index for HISAT2 by calling hisat2-build with...Last edited by dekontrol; 06-22-2017, 10:46 PM. -
Indexing- Exons and splice sites
Hi,
I have been following the github tutorial, https://github.com/griffithlab/rnaseq_tutorial/wiki
to learn RNAseq.
... -
Concentrations of Post-indexed libraries
Hello all
I have been working with microbiome libraries using Illumina's recommended protocol and sequencing the V3-V4 regions. My question... -
Cycle number? Indexing/enrichment NEBNext
I'm preparing dual-indexed libraries using NEBNext Ultra Library Prep for targeted resequencing on Illumina MiSeq and can't decide on the number of PCR... -
library prep using sonicator - indexing
I'm currently using Nextera XT to prepare libraries for MiSeq. This involves 'tagmentation' which simultaneously fragments (enzymatically) and adds a... -
library prep using sonicator - indexing
I'm currently using Nextera XT to prepare libraries for MiSeq. This involves 'tagmentation' which simultaneously fragments (enzymatically) and adds a... -
MiSeq - minimum index diversity?
Hi, we want to do a MiSeq run of a library pool of TruSeq DNA PCR-free (single index; 6 bp), and custom 16s amplicons (dual index 8+8 bp). We wish the... -
Flowcell with single and dual indexing demultiplexing issue
Hello,
I have received from my lab one HiSeq flowcell with 7 lanes single indexed and the 8th is dual indexed.
Something... -
Samtools indexing problems
Hi there!
I'm still pretty new to bioinformatics and using programs in Terminal (MacOS 10.9) and have been struggling to get samtools indexing...
