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  • Anojan
    Junior Member
    • Nov 2023
    • 1

    #1

    Indexing -bowtie2, aligning - STAR and FATAL ERROR

    Ciao,

    I'm a Bioinformatics newbie encountering an issue with STAR alignment. I downloaded the mm10 genome and indexed it with Bowtie 2. When attempting to run mapping with STAR, I received the following error message.

    "FATAL ERROR: could not open genome file /storage/data/SCA/Anojan/genomes/mm10/indexed_genomes//genomeParameters.txt SOLUTION: check that the path to genome files, specified in --genomeDir is correct and the files are present, and have user read permsissions while running start after indexing"

    But I couldn't find any genomeParamters.txt files. I think they are specific to the STAR indexing.

    How to fix it ?
    ​

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