Hi,
We've got the done the RNA-seq of a species (genome is about 3GB), but NO reference genome is available, using pacbio and Illumina. After the...
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how can I get abundance from both Iso-seq output and illumina data?
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Why the pacbio isoseq data used for RNA analysis is much more than illumina data?
As we all know, for the human RNA-seq analysis, 4-6G clean data from illumina platform is enough. But when I use pacbio's ISO-seq pipline, the subread... -
How much of PacBio ZMW Loading Productivity 1（p1） is best for IsoSeq?
Hi,
Now I am investigateing the IsoSeq in PacBio platform. I find that the ZMW Loading Productivity 1(P1) varies a lot from different experiments.... -
How can I calcualte the sequence identity and coverage form GMAP output bam file?
Now I have got a bam file from gmap alignment with data from pacbio isoseq result. Now I want to get the statistic information about the coverage and...Last edited by huan; 01-20-2016, 12:37 AM. -
Does the full length sequencing using Iso-Seq get the real full length ?
I have used pacbio SMRT Iso-seq module to analyze my cDNA sequencing data, including Getting full length reads, Isoform level clustering (ICE and Quiver),... -
How to run TOFU analysis in the perl ?
Now I am working on the Iso_seq Analysis using the Tofu program, I am intended to intergrated all the analysis (including classify,cluster,Removing Redundant... -
How can I get the report by using SMRT command-line version?
Now I have RS_IsoSeq command-line installed, and it works well for filter(getting reads of insert) classify(Getting full length reads) ,Isoform level... -
Identify Translated Sequence in Batch
Does anyone have any suggestions on how to identify in batch for isoseq data the start and stop positions for predicted gene sequence?
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Is There Any Tools/Methods For PacBio IsoSeq SNP/Fusion Genes Analysis?
Hi, there!
I have used pacbio SMRT Iso-seq module to analyze my cDNA sequencing data, including Getting full length reads, Isoform level clustering... -
how to extract alternative splicing information from Iso-seq nonredundant sequence?
I have used pacbio SMRT Iso-seq module to analyze my cDNA sequencing data, including Getting full length reads, Isoform level clustering (ICE and Quiver),...
