How much of PacBio ZMW Loading Productivity 1（p1） is best for IsoSeq?

[huan]

Hi,
Now I am investigateing the IsoSeq in PacBio platform. I find that the ZMW Loading Productivity 1(P1) varies a lot from different experiments. So I am confused what's the normal range for P1. And what's the effects for too low or too high P1? How does it come out for that?

Thanks a lot for any help!

[Magdoll]

First, our recommendation for loading for different applications can change over time, so please always check with your local PacBio FAS or sales person to get the most up-to-date answers.

Currently, for RS2 data, we recommend loading P1 between 50-70%. Some customers have had success loading above 70% but in-house we observe decline in base quality after 70%. Lower P1, by the way, does not necessarily improve base quality, but you get less yield and less bang for the buck, that's all

[huan]

Now we have a lab the loading p1 has been above 70%:
Prod=0 ZMWs: 9.675%
Prod=1 ZMWs: 76.32%
Prod=2 ZMWs: 14.01%
Total Active ZMWs: 90.32%
I can not figure out it is good or not. Is there any way to evaluate the quality of the data?


I have evaluated data quality using StsPlots Tool. But I don't understand what does the fiugur mean. So is there any one can help to tell is there any problem in my cell?

Thanks a lot for and advice!