Hi all,
we don't have the original aliquote of an old sample, but we have an Illumina Nextera Flex library in the freezer.
I am...
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Nanopore library on top of an Illumina library
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RADseq library Prep- Newer protocols for pooled samples?
Hello All,
I am hoping to use RAD-seq for the discovery of SNPs for a population genomics investigation. I have a few questions related... -
troubleshooting agilent bioanalyzer error
I ran an RNA 6000 nano chip with RNA samples in all 12 wells, some randomly came back flagged with error: unexpected signal in fast region. Is there any... -
Low input of primers for pre optimized PCR step
Hello,
I have created a situation where I only have enough reagents to add approximately 1/5 of the primers recommended (pre optimized by Illumina)... -
Nextera Rapid Capture Indexing
Hey there,
We have a 3-plex Nextera rapid capture Kit, and we want to run 6 captures in one NextSeq Run.
It is possible to use Nextera... -
How fast is your NGS library preparation?
Everyone wants NGS library preparation to be easy, simple, and fast. The reality is that protocol times for NGS library prep kits vary from vendor to... -
Low yield after library prep
Hello,
I am using the Ion Shear Plus reagents kit to prepare libraries for the PGM. I input 200 ng of DNA (measured by Qubit) but am only... -
Robotics Workstation: Sciclone NGSx vs QIAGEN suite
Hi,
We are looking into buying a robotics workstation system to perform high-throughput DNA/RNA extractions, but also preferably downstream...Last edited by BioGenomics; 02-02-2015, 06:09 AM. -
AT rich region depletion
We are having some trouble with our ChIP-seqs: we seem to be completely blind to AT rich regions. When I check the GC distribution in the ChIP and Input... -
The best company for library construction
Hi all,
We are planning to run RNAseq experiments. Could someone advise which company today offers library construction of a very good quality... -
FFPE DNA Repair
Hi,
I am working on optimizing FFPE extraction for our next generation sequencing applications. I am currently interested in trying to... -
Labs that have tried with CLIP-PE
Hi guys,
Do you know any lab that has experience working on Illumina library construction using CLIP-PE methodology (Peng Z, Zhao Z, Nath... -
CG content and Illumina Sequencing
Hi,
We are having some problems sequencing DNA regions with a GC% higher than 75 in a bacteria with a GC average content of 67%. We are... -
Wouldn't an RNase+ reverse transcriptase give better library representation?
Hi All,
I will be building a library for mRNA Seq where I want to count transcripts (measure gene expression). Wouldn't using a reverse transcriptase... -
pre-small RNA library prep
I am attempting to prep a small rna library and have heard of people selecting for the small rna prior to introduction into protocols. I was wondering...
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