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PCR validation of ATAC-seq library
I have performed PCR validation of an ATAC-seq library targeting known/expected accessible chromatin regions (100bp) but 90% of the products have a smearing...Last edited by MzwaneleN; 10-09-2020, 02:12 PM. -
Contamination of E.coli reads in ATAC-seq library
Hi All,
I have recently run an ATAC-seq library on PBMCs that came back with 82% reads mapping to E.coli and only 5% mapping to the human... -
Ribodepletion: RiboZero kit (smaller rxns?)
We are planning an RNA-seq experiment requiring rRNA depletion (in Xenopus laevis). Previous literature has shown success with our system using the Illumina... -
Application Scientist NGS at MPI of Biochemistry, Munich, Germany
The Max Planck Institute of Biochemistry in Martinsried - near Munich- is one of the leading institutes in the molecular life sciences, with about 30... -
Question about library normalization methods
Hi all,
I am looking for a bead based library normalization method as an alternative to the library normalization in the NexteraXT kit.... -
the second strand cDNA synthesis in RNA-seq library generation
Hi everyone,
i am in the middle of RNA-seq now, and i wonder what are the ways to synthesize the second strand cDNA currently in RNA-seq library... -
Multiplexing RRBS samples
Dear everyone,
Im preparing a RRBS library. I want to multiplex 10 samples from 150ng DNA.
We use ilumnia DNA nano LT adaptors... -
Required oligo purity for homemade library preps?
Hi, we are gearing up to make some home-made libraries with custom oligos. I have never ordered oligos for library construction, what are the purity requirements?... -
Shearing ssDNA
I'm trying to prepare RNA samples for 3' end mapping, and would like to shear them at the ssDNA stage after first strand synthesis. Does anyone know... -
Library QC- cntrl loci enrichment doesn't match pre and post library generation
Hi all- my first post, so please be kind if I miss some expected content.
Why doesn't my post amplification enrichments match my pre amplification... -
TruSeq DNA 500bp library prep
Hello, we are planning to make a TruSeq library with an starting insert of 500bp and worried that this higher molarity might affect the subsequent TruSeq... -
Multiplexing Transcription factor Chip-seq
Hi all,
We will be soon starting to do libraries from Transcription factor (TF) Chip-seq, for a genome of ~ 330 Mb.
We want... -
RNA-seq libraries from bird blood
Hi all,
I am planning a RNASeq experiment and I would like to prepare some Illumina libraries. The starting material is avian blood. I had a look... -
default RNA-Seq library prep
What with all the developments in the RNA-Seq library prep field our lab is contemplating moving away from the Illumina Tru-Seq RNA kits and using the... -
Fluidigm PCR amplicon Library prep
Hi has any one got any advice on a two-step PCR amplicon library generation via barcode/adapter addition using a secondary PCR.
I am...
